Augmentation in biological pest control involves the supplemental release of natural enemies. Relatively few natural enemies may be released at a critical time of the season (inoculative release) or literally millions may be released (inundative release). Additionally, the cropping system may be modified to favor or augment the natural enemies. This latter practice is frequently referred to as habitat manipulation. An example of inoculative release occurs in greenhouse production of several crops. Periodic releases of the parasitoid, Encarsia formosa, are used to control greenhouse whitefly, and the predaceous mite, Phytoseiulus persimilis, is used for control of the two-spotted spider mite. Lady beetles, lacewings, or parasitoids such as trichogramma are frequently released in large numbers (inundative release). Recommended release rates for Trichogramma in vegetable or field crops range from 5,000 to 200,000 per acre per week depending on level of pest infestation. Similarly, entomopathogenic nematodes are released at rates of millions and even billions per acre for control of certain soil-dwelling insect pests. Habitat or environmental manipulation is another form of augmentation. This tactic involves altering the cropping system to augment or enhance the effectiveness of a natural enemy. Many adult parasitoids and predators benefit from sources of nectar and the protection provided by refuges such as hedgerows, cover crops, and weedy borders.

Mixed plantings and the provision of flowering borders can increase the diversity of habitats and provide shelter and alternative food sources. They are easily incorporated into home gardens and even small-scale commercial plantings, but are more difficult to accommodate in large-scale crop production. There may also be some conflict with pest control for the large producer because of the difficulty of targeting the pest species and the use of refuges by the pest insects as well as natural enemies. Examples of habitat manipulation include growing flowering plants (pollen and nectar sources) near crops to attract and maintain populations of natural enemies. For example, hover fly adults can be attracted to umbelliferous  plants in bloom. Biological control experts in California have demonstrated that planting prune trees in grape vineyards provides an improved overwintering habitat or refuge for a key grape pest parasitoid. The prune trees harbor an alternate host for the parasitoid, which could previously overwinter only at great distances from most vineyards. Caution should be used with this tactic because some plants attractive to natural enemies may also be hosts for certain plant diseases, especially plant viruses that could be vectored by insect pests to the crop. Although the tactic appears to hold much promise, only a few examples have been adequately researched and developed.

Ladybugs, and in particular their larvae which are active between May and July in the northern hemisphere, are voracious predators of aphids such as greenfly and blackfly, and will also consume mites, scale insects and small caterpillars. The ladybug is a very familiar beetle with various colored markings, whilst its larvae are initially small and spidery, growing up to 17 mm long. The larvae have a tapering segmented grey/black body with orange/yellow markings and ferocious mouthparts. They can be encouraged by cultivating a patch of nettles in the garden and by leaving hollow stems and some plant debris over winter so that they can hibernate. Hoverflies resemble slightly darker bees or wasps and they have characteristic hovering, darting flight patterns. There are over 100 species of hoverfly whose larvae principally feed upon greenfly, one larva devouring up to fifty a day, or 1000 in its lifetime. They also eat fruit tree spider mites and small caterpillars. Adults feed on nectar and pollen, which they require for egg production. Eggs are minute (1 mm), pale yellow white and laid singly near greenfly colonies. Larvae are 8-17 mm long, disguised to resemble bird droppings, they are legless and have no distinct head. Semi-transparent in a range of colours from green, white, brown and black.

Hoverflies can be encouraged by growing attractant flowers such as the poached egg plant (Limnanthes douglasii), marigolds or phacelia, throughout the growing season. Dragonflies are important predators of mosquitoes, both in the water, where the dragonfly naiads eat mosquito larvae, and in the air, where adult dragonflies capture and eat adult mosquitoes. Community-wide mosquito control programs that spray adult mosquitoes also kill dragonflies, thus removing an important biocontrol agent, and can actually increase mosquito populations in the long term. Other useful garden predators include lacewings, pirate bugs, rove and ground beetles, aphid midge, centipedes, predatory mites, as well as larger fauna such as frogs, toads, lizards, hedgehogs, slow-worms and birds. Cats and rat terriers kill field mice, rats, june bugs, and birds. Dogs chase away many types of pest animals. Dachshunds are bred specifically to fit inside tunnels underground to kill badgers.